Genetic characterisation and associated endosymbionts of insect pest Bemisia tabaci nourishing on mungbean

Abstract

Current study outlines the incidence of bacterial endosymbionts and the genetic group of Bemisia tabaci nourishing on mungbean plantation in New Delhi, India. Genetic group analysis based on mitochondrial cytochrome oxidase 1 (mtCO1) gene presented the presence of Asia II 1 genetic group on mungbean plantation. Individuals were examined for symbiotic bacterial infection using specific primers amplifying the 16S rRNA gene for Rickettsia, Cardinium and Wolbachia, and the 23S rRNA gene for Arsenophonus. The primary endosymbiont, Portiera aleyrodidarum was present in all the examined samples and a disproportion was detected in the incidence frequency of secondary endosymbionts. The figures of unbalanced incidence of secondary endosymbionts and genetic group of B. tabaci conveys the elementary data of this notorious pest for progressive studies on the control measures of this insect pest over mungbean plantations.