Efficiency of thawing cum controller unit against normal water bath for determining the cattle sperm quality parameter test

Abstract

Artificial insemination (AI) is the first generation reproductive biotechnology that has made a biggest contribution to the genetic improvement, particularly in dairy cattle. Frozen semen in straws has become the universally accepted unit of storage and transfer of bovine genetics to cattle procedures which depends on preserve the functional activity of spermatozoa (viability and fertilizing ability) [1]. High viability and motility of spermatozoa are important factors for successful artificial insemination (AI) because a significant correlation between post-thawing sperm viability and subsequent conception rate has been reported [2]. Many techniques are there in India to thaw frozen semen straws before insemination which not only affecting the motility of sperm it also affect the sperm morphology ex Acrosome intactness. For this a study was conducted in which 15 bulls and buffaloes frozen semen straws are thawed and tested in different commercially available portable thawing kit in compare to normal water bath and results are found to be satisfactory. There was non-significant difference in between sperm quality parameters thawed in all the three thawing unit and thawing cum controller can be used in the farms where the artificial inseminations processes are carried out.