Ninhydrin staining and SDS PAGE analysis revealed variations in egg shell surface and water soluble egg shell proteins in a few Avian eggs

Abstract

The present work was aimed at analyzing the protein of egg shell and its membrane involving three methodology yielded a confirmative results. The ninhydrin stained egg shell surface had shown positive rheumann purple coloration in three species Gallus gallus domesticus (Poultry chicken), Gallus gallus (Native chicken) and Griraja chicken but not in Columbia livia (pigeon). The shell pores were clearly visible in three egg shell species of family Galidae, but in Columbia livia pores appeared as dented depression on the shell. The qualitative estimation of shell protein showed variation in the quantity. Least Quantity of 0.02mg/100 mg in Gallusgallus domesticus and highest quantity of protein 0.25mg/100 mg in Columbia livia was recorded. The electrophoretic analysis of water soluble protein manifested different in all the four species, of these highest molecular weight of 63.09 KDa and 44.04KDa recorded in columbialivia that had two types of protein. In Giriraja and Gallus gallus species single band of protein having molecular weight 39.21KDa and 44.05KDa represented respectively. In Gallus gallus domesticus two bands of lower molecular weight of 21.98 KDa and 8.91 KDa were recorded. This study has confirmed the importance of Ninhydin staining in the study of egg shell profile as well deciphered the existence of water soluble protein in egg shell.