Collection, isolation and bioassay studies of indigenous isolates of Lecanicillium lecanii (Zimm.) Zare and Games against Myzus persicae (Sulzer)

Abstract

The evaluation of indigenous local isolates of Lecanicillium lecanii was determined against Myzus persicae by detached leaf smeared method. A total of three isolates of L. lecanii from the 30 samples were obtained based on their pathogenicity test against M. persicae. Among the three isolates R7BGBD (100%) and R8GAR (96.67%) showed highest mortality whereas the least mortality was found in the isolate R4HAT (56.67%) at eight days after treatment at the concentration of 2 x 10 9 cfu/1000 ml. The isolate R7BGBD was the most virulent at six and eight days after treatment, the LC50 values being 0.54 X 109 and 0.15 X 109 cfu/1000 ml, respectively. The order of virulence based on LC50 values was R7BGBD> R8GAR> R4HAT. These isolates performed better under laboratory conditions and need to be evaluated under field conditions for their promotion against sucking insect pests of various Horticulture and Agricultural crops.