TPC, TFC & Antioxidant activities of Crepidium acuminatum (D. Don) Szlach

Abstract

Crepidium acuminatum D. Don Szlach is an important medicinal plant belonging to family Orchidaceae. This plant is an important part of Astavarga used in nutritional preparation. Only a few papers have been reported in scientific database. We focussed on estimation of total phenolic content, total flavonoids content and in vitro antioxidant potentials by three methods i.e. reducing power method, Total antioxidant activity by Ammonium molybdate method and Ferric Reducing Antioxidant Powder method. The phenolic content with respect to gallic acid was found to be 70.9 ± 0.53 (µg gallic acid equivalent/ml of extract) for methanol extract. The flavonoid content was found to be as: 34.27 ±0.61 (µg quercetin equivalents/ml of extract) in methanol extract Three types of extracts were used for anti-oxidant studies: aqueous, hydroalcohlic and alcoholic. It was concluded that aqueous extract has more potential as antioxidant as compared to hydroalcolic and alcoholic extracts.