In vitro cytotoxicity study of leaf, stem and bark of Pterocarpus santalinus Linn. F.

Abstract

The leaf, stem and bark of Pterocarpus santalinus belonging to the family Fabaceae were evaluated for their in vitro cytotoxicity potential by MTT assay. The plant parts were extracted by individual and successive cold percolation method using a number of solvents of varied polarity. The extractive yield varied with different solvents. The cytotoxic study of methanol extract of all the three parts extracted by both the methods was done against HeLa cancer cell line, breast cancer cell line and normal cell line. Mitomycin C was used as positive control and DMSO as negative control. All the three extracts showed dose dependent cytotoxic effect. When all the three parts were compared, ME extract of stem showed minimum % cell viability and both methods were compared, successive extraction methods was better than individual extraction method. Though there was not much difference in % cell viability by either method or by different parts. So it can be concluded that all the three parts showed cytotoxic effect against HeLa and breast cancer cell line.