Antimicrobial and antiradical activities of ethyl acetate extracts from endophytic fungi isolated from Cameroonian medicinal plants

Abstract

The antimicrobial activity of extracts from endophytic fungi were determined using microdilution method. The 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was used for antiradical activity and the chemical profile of promising extracts was analyzed using HPLC-DAD, UPLC/MS-QTOF and HPLC/MSn Electrospray Ion Trap. The Minimum Inhibitory Concentration on yeasts and bacteria ranged from 0.019 to 5 mg/mL. Extract from T. atroviride was most active against yeasts (MIC from 0.019 to 5mg/ml) and P. chermesinum (MIC from 0.625 to 0.019 mg/ml) was against bacteria. The best radical scavenging activity was found with Diaporthe sp extract (%I from 9.929 to 41.134%). The chemical composition of extracts from T. atroviride, P. chermesinum and Diaporthe sp showed the presence of 8, 12 and 10 compounds respectively amongst which Genipinic acid, Citreomantanin, Dermoglaucin, Alternariol-5-methylether, Ascochitine, Aflatoxicol, Ascorbic acid, 4-deoxybostrycin, Bionectriamide B, Nivalenol, and Trisdechloronornidulin were identified. These results indicate that metabolites produced by endophytic fungi found in Cameroonian medicinal plants are potent source of bioactive compounds.