Remedial effects of exogenous surfactant in Pseudomonas aeruginosa LPS-induced acute lung injury in rat model

Abstract

The present study was planned to evaluate the effects of exogenous surfactant in acute lung injury induced by Pseudomonas aeruginosa lipopolysaccharide. Fifty adult male Wistar rats were obtained from faculty of veterinary medicine, Zagazig University, Egypt, during March 2017 and assigned to five equal groups. Animals in the second, third, fourth and fifth groups received a single dose of 0.2 mL of 0.9% normal saline, 0.2 mL of survanta, LPS (5 μg/kg bw), and both survanta and LPS respectively. Exogenous surfactant administration significantly reduced the serum levels of IL-1ᵦ, IL-6, IFNγ, and TNFα from 333.32±14.02, 273.55±10.22, 404.05±10.45 and 364.27±17.26 in LPS group to 179.77±17.05, 185.92±12.78, 300.69±7.81 and 262.08±10.97 respectively. Also, it significantly reduced the values of integrated density of NF-KB p65 pulmonary expression from 348.02±9.30 in LPS group to 167.40±7.29. The pulmonary lesions were markedly ameliorated in the surfactant treated group when compared to the LPS group.