Analysis of bioactive compounds in Physalis minima leaves using GC MS, HPLC, UV-VIS and FTIR techniques

Abstract

The bioactive components of Physalis minima leaves have been evaluated using GCMS, HPLC, UV VIS and FTIR. The chemical compositions of the extract of Physalis minima leaves were investigated using Perkin-Elmer Gas Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched by the National Institute of Standards and Technology (NIST) library. GC/MS analysis of extract of Physalis minima leaves revealed the existence of Heneicosanoic acid (25.22), Bicyclo [4.1.0] Hepta-2, 4-dien (27.41) Octadecanoic acid (CAS), Stearic acid (31.19) and Octadeca-9, 12-dienoic acid (32.02). HPLC profiles of Physalis minima reported to contain four phenolic compounds, namely Ellagic acid (4.13 min), Catechol (3.59 min), Gallic acid (4.12 min) and Catechin (7.41 min). The UV- VIS profile showed the peaks at 315.09 nm, 408.09 and 676.50 nm with the absorption 0.247, 0.106 and 0.003 respectively. The results of FTIR analysis confirmed the presence of phenol, alkanes, aldehyde, secondary alcohol, amino acid, aromatic amines and halogen compound. The results of this study offer a platform of using Physalis minima leaves as herbal alternative for various diseases.