HPTLC fingerprint of 85% methanolic extract of the root bark of Plumbago zeylanica Linn and it’s lethal dose estimation in mice

Abstract

The root bark of Plumbago zeylanica Linnhas potent analgesic and anti-inflammatory activity which has been used in many formulations of Indian system of medicine. The present studies investigated the quality of different solvent extracts from this plant and analyzed the safety of methanolic extract in mice. The qualitative studies for phytochemical constituents done on the extracts revealed the presence of more constituents such as polyphenols, sterols, CHO and proteins in the methanolic extract of this plant among other extracts and also this extract yield more. The HPTLC fingerprint of this extract shows the presence of seven compounds with high concentration at Rf 0.85. The methanolic extract was screened for acute toxicity study in rats and mortality was not found up to the dosage of 4000 mg/kg b.wt. By Litchfield and Willcoxan method, LD50 estimated more than 4000 mg/kg and ED50 found from 200 mg/kg to 400 mg/kg b.wt.