Preliminary phytochemical study and HPTLC valuation of leaves of grey mangrove Avicennia marina

Abstract

Mangroves are the chemical factories of nature and extensive sources of organic chemical matter on this planet. The conventional methods are poor, time consuming as well as less systematic, so there is a need to use appear technological knowledge and sophisticated scientific methods to fulfill this knowledge gap. Therefore, present study is an attempt to study the preliminary phytochemistry and distinguish HPTLC pattern of Avicennia marina leaf. To check presence or absence of photochemical compounds like alkaloids, flavonoids, tannins, terpenoids, saponins. reducing sugar, phenols, steroids and glycosides were identified by preliminary analysis. Toluene: Ethyl Acetate: Formic acid: Methanol: 7:5:1:0.5 as solvent system is the best solvent system for measuring the establishment of phytochemicals of A. marina. HPTLC analysis of methanol extract showed nine peaks and the Rf values ranged in between 0.07 to 0.80 at 254nm and Rf values ranged in between 0.07 to 0.81 showing six peaks at 366 nm. It is used as a phytochemical identification tool to check genetic variability in various plant species.