Antimicrobial, antioxidant and anti-hemolytic effect of different fractions of ethanolic extracts of Tamarindus indica L. bark

Abstract

This study has conducted an investigation on evaluation of antimicrobial, anti-hemolytic and antioxidant activity of different fractions ethanolic extract of Tamarindus indica L bark against pathogenic bacteria, fungi. Four different Species of Gram-positive and Gram-negative bacteria, four species of fungi, Proteus hemolysin are used in this study. Tamarindus indica L. is widely investigated but limited information is available on its bark (Ti-b). Ti-b was studied to combat against uropathogen Proteus for anti-hemolytic effect, isolated from UTI patient’s catheter. Ethanol fractions of each specimen were tested for antimicrobial and antioxidant study. Active specimen was partitioned with TLC method to determine bioactive compound. Hemolysis caused by secreted hemolysins of Proteus was prominent (75%). Phenolic compound in Ti-b potentially inhibited that hemolysis. Phenolic compound(s) in Ti-b enabled to deactivate Proteus hemolysins and thereby strongly inhibited hemolysis. Ti-b show strong antioxidant property.