Study on antioxidant and antimicrobial activities of methanolic leaf extract of Glycosmis pentaphylla against various microbial strains

Abstract

The purpose of the present study was to investigate the antioxidant and antimicrobial potential of methanolic extract of Glycosmis pentaphylla (family: Rutaceae) leaf which possesses significant drug value. In vitro antioxidant activity was performed using DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging activity and total phenol content by Folin–Ciocalteu Reagent. The antioxidant activity of different concentrations of plant extracts were found to increase in a concentration dependent manner where the IC50 value was 69.64 i­g/ml compared to the ascorbic acid (27.02 i­g/ml). The total phenol content was found in plant extract was 25.28 mg/g GAE. The antimicrobial assay was performed by disc diffusion method using kanamycin and nystatin as the standard. The highest antimicrobial action was found to be against Salmonella paratyphi (zone of inhibition with 22 mm). The MIC was carried out by the serial dilution technique where it showed highest inhibitory effect with minimum concentration of 16 i­g against Salmonella paratyphi and Escherichia coli. The obtained results indicate that investigated plant could be potential sources of natural antioxidants and antimicrobial agents and can be used in the treatment of oxidative disorders and many infectious diseases caused by microorganisms.