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A reference genome for Nicotiana tabacum enables map-based cloning of homeologous loci implicated in nitrogen utilization efficiency

BMC Genomics · 2017 · Vol. 18(1) · pp. 448–448

Kieron D. Edwards✉(British American Tobacco (United Kingdom))Noé Fernández‐Pozo(Cornell University)Katherine E. Drake-Stowe(North Carolina State University)M. Humphry(British American Tobacco (United Kingdom))Andrew Evans(British American Tobacco (United Kingdom))Aureliano Bombarely(Virginia Tech)F H Allen(British American Tobacco (United Kingdom))Rachel Hurst(British American Tobacco (United Kingdom))Benjamen White(British American Tobacco (United Kingdom))Sheri P. Kernodle(North Carolina State University)Jennifer R. Bromley(British American Tobacco (United Kingdom))J. P. Sanchez-Tamburrino(British American Tobacco (United Kingdom))Ramsey S. Lewis(North Carolina State University)Lukas A. Mueller(Ithaca College)

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Abstract

Development of an improved genome assembly for N. tabacum enables what we believe to be the first successful map-based gene discovery for the species, and demonstrates the value of an improved assembly for future research in this model and commercially-important species.

Plant nutrient uptake and metabolism Genomics and Phylogenetic Studies Legume Nitrogen Fixing SymbiosisBiologyNicotiana tabacumGenomeSequence assemblyGeneticsGeneComputational biologyNicotianaTranscriptomeGene expression