Carotenoid rodoxanthine obtained from the seeds of Ricinus communis act as potential hepatocytes regenerators

Abstract

The liver is the only visceral organ that possesses remarkable capacity to regenerate. The liver can regenerate after either surgical removal or after chemical injury. It is known that as little as 25% of the original liver mass can regenerate back to its full size. The process of regeneration in mammals is mainly compensatory growth because only the mass of the liver is replaced not the shape. Liver regeneration involves replication of the liver cells, mainly hepatocytes, followed by other cells such as biliary epithelial cells and sinusoidal endothelial cells. Once cell proliferation is completed, the newly divided cells undergo restructuring, angiogenesis and reformation of extracellular matrix to complete the regeneration process. The main objective of present research work is to screen the bio molecules present in EE-CS and isolate the bioactive carotenoid rodoxanthine and evaluate the in vivo hepatocytes regenerators’ potentiality. Characterization of rodoxanthine was carried out by U.V, IR, 1H-NMR, 13C-NMR, Mass spectra and HPLC etc. The in vivo hepatocytes regenerator’s potentiality was performed against CCl4 induced rat hepatocytes. The results obtained from the in vivo experimental data had shown that the elevated levels of SGOT, SGPT, ALP and Serum bilirubin due to CCl4 intoxication were reduced significantly (*P