Efficiency of Bacillus thuringiensis var krustaki with Henna essence and Neemarin (Neem toxicant - Azadirachta indica A.) on second and third instars larvae cabbage large white butterfly Pieris brassicae L. (Lep: Pieridae) in Lab conditions

Abstract

Cabbage large white butterfly Pieris brassicae L. is one of the most important pests of cabbage crops in Urmia city of west Azerbaijan province. According to risks of pesticide residue and environmental damage using biological control agents, especially Bacillus thuringiensis is considered. In the current research, the effect of B. thuringiensis, Neemarin and Henna essence in separately and combination form on second and third instars larvae was investigated. The result show that combined effects three agents on second and third instars larval stages was additive. Percentage of mortality mixture of bacteria, Neemarin and Henna treatments was increased in compare bacteria, Neemarin and Henna alone, So that the average mortality combined three agent treatment on 2nd and 3rd instars larvae after 24, 48 and 72 hours were (56.66, 63.33 and 66.66%) and (50, 56.66 and 60%), respectively. Our study indicated that the integrating B. thuringiensis, Neemarin and Henna as environmentally acceptable agents has opened further opportunities for their uses as integrated management of this pest.