Development of Standardisation parameters and Isolation of Phytomarker Myricetin from stem bark of Myrica esculenta Buch. Ham. Ex d. Don

Abstract

The present study was undertaken to develop standardization parameters and to develop method for isolation of phyto marker myricetin from the stem bark of Myrica esculenta Buch. Ham. Ex D. Don, Myricaceae. Different pharmacognostical and phytochemical parameters for quantitative estimation of phytoconstituents and separation of marker myricetin from the methanolic extract by column chromatography were studied. The stem bark of M. esculenta revealed 2.47% of total ash, 0.02% of acid insoluble ash, 24% of water soluble extractive, 13% of alcohol soluble extractive, 9.9% of moisture content and foaming index of 125. Preliminary phytochemical screening revealed that the stem bark was credited with phytoconstituents like tannins, phenols, flavonoids and saponins in methanolic and aqueous extract. Stem bark contains 0.53%w/w of total flavonoid while the total phenolic content was found to be 9.18%w/w.43 fractions were isolated by column chromatography. Thin layer chromatographic studies of isolated fractions resulted in separation of yellow colored spot at the Rf value 0.65. The U.V. spectroscopic studies of isolated spots produced peaks at 254 and 375 nm which were similar to the peaks of the reference compound myricetin.