Analytical Profile and In Vitro Antimicrobial Activity on leaves of Marsilea minuta Linn. (Marsileaceae)

Abstract

To establish the fingerprint profile of Marsilea minuta Linn. using thin layer chromatography (TLC) and high performance thin layer chromatography (HPTLC) technique and to investigate the antibacterial potentials of leaves of Marsilea minuta Linn. The TLC study was carried out in two solvent systems, which showed different Rf value and HPTLC study was carried out using methanol extract, compared with reference standard (i¢-sitosterol). The antibacterial activities of extracts of Marsilea minuta were tested against some medicinally important bacterial strains. The antimicrobial activity was determined in the extract using agar disc diffusion method. These plants can be used to discover bioactive natural products that may serve as route in the development of new pharmaceuticals research activities.