In vitro antidiabetic activity of methanolic extract of Citrus limon, Punica granatum, Musa acuminata peel

Abstract

Diabetes is a clinical syndrome characterized due to absolute or relative deficiency of insulin. The intestinal digestive enzymes play a vital role in the carbohydrate digestion. Recent decades have experienced a sharp increased in the incidence and prevalence of diabetes mellitus. One antidiabetic therapeutic approach is to reduce gastrointestinal glucose production and absorbance through the inhibition of carbohydrate digestive enzyme such as alpha-amylase. Medicinal plants have been reported to play an important role in modulating glycemic responses and have preventive and therapeutic implications. The aim of the current study was to evaluate the methanolic extracts of three kinds of fruit peels (Lemon, pomegranate and Banana) for its in vitro antidiabetic activity. Our result suggests that methanol extracts of all three extract exhibildose-dependent increase in percentage inhibitory activity on alpha amylase enzyme. Acarbose was used as a standard drug. Maximum alpha amylase inhibitory activity from banana peel was found to be 80.87% at 1000µg/ml. The Ic50 values of alpha amylase inhibitory activity of Lemon, Pomegranate, and banana were found to be 135.354%, 157.928% and 185.384% respectively. The findings indicate banana peel was found to be more potent and posses hypoglycemic effect and hence for the management of diabetes mellitus can be utilized as complement.