Evaluation of acute and sub-acute toxicity effect of ethanol extract from Caesalpinia bonducella seed kernel.

Abstract

Seed kernel of Caesalpinia bonducella comprises of various influential components. The ethanol extract of these seed kernels have potential antibacterial activity against pathogenic micro-organisms. The antibacterial activity is more prominent against fungi. The acute toxicity studies of extract in albino mice were investigated. Various doses of extract from 5 to 2000 mg/kg bodyweight were administered orally to the test groups of mice, while distilled water was given to the control group to evaluate the toxicity in mice after ingestion of the extract for one day (acute model) and for 14 days(sub-acute model). The histopathological studies of liver, heart, and kidney indicated either no or less alteration in the treated group. However, a slight change in liver cellular architecture was observed with tubular congestion, tubular desquamation, glomerular congestion and interstitial inflammatory cells of test animal. The results indicated that LD50 of the extract is higher than 2000 mg/kg and no changes were observed in any behavioral parameters in mice.