Isolation and Purification of Lectin from Soybean (Glycine Max) Mahabeej Js-335

Abstract

Plant lectins have a wide spectrum of biological significance. In this project work the isolation and purification of the lectins (SBL) from the Soybean (Glycine max) Mahabeej JS-335 have been reported. The lactose binding lectins were purified from the Soybean seeds by performing affinity chromatography using a lactamil sephadex 100G. Affinity products were dialysed against PBS and SDS-PAGE was done to identify the fractions ‘molecular weight. This work explored different techniques of protein (lectin) purification and describing their characteristics.