GC-MS profiling and in vitro radical scavenging effect of Adhatoda beddomei

Abstract

To investigate the in vitro radical scavenging assays and phytochemical identification of an ethanol leaf extract of Adhatoda beddomei (AB). The fresh leaves of AB (1000 gm) were shade dried at room temperature for 30 days and the dried leaves was made into a fine powder. The phytochemical screening studies identified fourteen chemical constituents present in the leaf extract of AB. The ethanol extract of AB exhibited a significant dose dependent inhibition of in vitro radical scavenging assays and their corresponding IC50 values as follows such as Hydroxyl radical (48.10±0.49 µg/mL), Superoxide anion (34.50±0.49 µg/mL), Nitric oxide radical (49.11±0.49 µg/mL), DPPH radical (50.11±0.50 µg/mL) and Reducing power (16.80±0.16 µg/mL). Thus, our results show that AB exhibits various in vitro radical scavenging assays and possess important bioactive phytocompounds such as squalene and vitamin E, campesterol, stigmasterol, I²-Sitosterol and lupeol.