Confirmation and optimization of Bhavana Vidhi in Tribhuvankeerti Ras - An Ayurvedic formulation

Abstract

The present study carried out to analyze the effect of bhavana in standardisation of Tribhuvankeerti Ras. An attempt has also been made to correlate the analytical test results with the efficacy study. The standardization of Tribhuvankeerti Ras was carried out using HPTLC and FT-IR. Also, the therapeutic efficacy of Tribhuvankeerti Ras was carried out on Swiss albino mice. The HPTLC fingerprint profile depicts the presence of all three bhavana dravyas Tulasi Ras, Adrak Ras and Dhatura Ras. The result showed that all the bhavanas in Tribhuvankeerti Ras formulation has exactly same Rf values as they had in alone and FT-IR analysis confirms unique transmittance peak in range of 4000cm-1 to 600cm-1. The pharmacological result elaborates the anti-pyretic effect of Tribhuvankeerti Ras in mice. The HPTLC and FT-IR methods developed for confirmation of bhavana dravyas in Tribhuvankeerti Ras will help in establishing the specifications and need for presence of bhavana dravyas.