Evaluation of Cytotoxic and Anthelmintic Activity of Plant Roots of Clerodendrum viscosum (Family: Verbenaceae)

Abstract

In the present study, in-vitro cytotoxic and anthelmintic activity of root extract of C. viscosum was evaluated. The Brine shrimp lethality bioassay method was used to determine the cytotoxic activity and vincristine sulphate was used as positive control. The plant extracts were also evaluated for in-vitro anthelmintic activity against Bangladeshi earthworms Pheretima posthuma and the time taken for worm paralysis and death was determined. One way analysis of variance (ANOVA) was used to determine the level of significance. The methanolic crude extract of plant root exhibited mild to moderate cytotoxicity with LC50 value of 10.235±1.061 mg/ml (95% CI: 7.60-12.87) as compared with vincristine sulphate with LC50 value of 0.841±0.025 mg/ml (95% CI: 0.78-0.90) and the difference was statistically significant (p