Comparison study between a crude bacterial suspension and purified pseudomonal alkaline protease enzyme effect on the rabbit interleukin – 2 following bacterial keratitis

Abstract

The present study was design to determine and evaluate the inhibitory effect of crude bacterial suspension and pseudomonas alkaline protease enzyme on the rabbit interleukin 2. Eight serum samples were collected from male rabbits; four rabbits were infected with a crude bacterial suspension of Pseudomonas aeruginosa at a dosage of 500µl. The remaining four rabbits were infected with pure pseudomonal alkaline protease enzyme by the same dosage bacterial suspension. Serum samples from two infected rabbits groups were examined by ELISA and used for the detection of the level of IL2. The results showed that the serum IL2 level of bacterial suspension treatment group significantly reduced by a mean of 14 ug/dl after treatment. The treatment effect in this group was evaluated as a strong effect. Whereas the serum IL2 level among the enzyme treatment group showed no statistically significant difference from a mean change of zero after treatment.