Presence of plasmid-R in isolated bacteria of oyster (Crassostrea virginica), during its process of collection, distribution, commercialization, and consumption

Abstract

The aim of the present study is to determine presence of Plasmid-R in isolated bacteria of C. virginica, during its process of collection, distribution, commercialization, and consumption in Alvarado, Veracruz lagoon. After being identified, strains were subjected to processes of: alkaline extraction of Plasmid-R; electrophoresis in agarose gel; and to susceptibly to antibiotics determination, through plate diffusion. 34 different species of pathogenic bacteria for humans were identified, belonging to Enterobacteriaceae, Vibrionaceae, Aeromonadaceae and Pseudomonadaceae families, from which a total of 519 Plasmid-R were obtained, 37 in recollection, 87 in distribution, 203 in sale and 192 in the phase of oyster consumption. There was no significant difference in number of plasmids between each phase, nevertheless, the increase in number of Plasmids-R through the process of commercialization is significant. In each phase it was registered bacteria with resistance to antibiotics like ampicillin, carbenicillin, cephalothin and nitrofurantoin.