Random amplified polymorphic DNA based characterization of flesh flies

Abstract

Flesh flies are medically important flies as they cause myiasis in livestocks. At an early stage of development these flies are morphologically identical. Therefore, Random Amplified Polymorphic DNA- Polymerase Chain Reaction (RAPD-PCR) technique was used to explore its importance as genetic marker for identification process. From the twelve primers used for RAPD-PCR method, different species specific bands were obtained, which can be further utilized for identification purpose and the data obtained support the RAPD-PCR methods ability to distinguish between two flies of the genus Sarcophaga i.e. S. albiceps and S. knabi. The mean heterozygosity observed in S. knabi was 0.150 and in S. albiceps it was 0.114, suggesting that there exists a low genetic variation in these species.