Antioxidant and urease inhibition activity of methanol extract of Hibiscus schizopetalus (Mast) Hook.

Abstract

The present study was aimed at measuring the antioxidant and urease inhibition capacities of methanolic extracts of flower and leaves (HFE and HLE) of Hibiscus schizopetalus (Mast) Hook (Malvaceae) an ornamental plant of this region. These extracts were screened for their scavenging properties using DPPH radical scavenging and nitric oxide antioxidant assay. Research on urease inhibitions from natural source yielded several imperative therapeutic drugs that are useful in gastric and urinary tract infections. Radical scavenging activity of extracts were evaluated at a concentration of 20 mg/ml, 40 mg/ml, 60 mg/ml, 80 mg/ml and 100 mg/ml using ascorbic acid as standard antioxidant. IC50 values for ascorbic acid and extracts (HFE, HLE) were 28 ± 0.04 mg/ml, 38.2 ± 0.08 mg/ml and 58.9 ± 0.13 mg/ml respectively. While 10, 25, 50, 100, 150 mg/ml contractions of extracts were used for nitric oxide scavenging activity. The maximum activity of extracts (HFE, HLE) was observed at 150 µg/ml (80.70% and 75.2%) respectively, whereas ascorbic acid exhibited 81.02% urease inhibition at the same concentrations. The percentage urease inhibition of standard Thiourea was found to be 98.2%, IC50 88.2 ± 0.01 mg/ml. HFE extract showed maximum urease activity with percentage inhibition of 55.5%, IC50 80.1 ± 0.87 mg/ml. While least inhibitory activity was observed in HLE extract i.e. 22.2%. This is the first report of mention research on Hibiscus schizopetalus (Mast) Hook in Pakistan. The results obtained in this study clearly showed that methanolic extracts of flower and leaves of plant could be proved as a good antioxidant and antiurease agent.