HPTLC finger printing analysis of the tannins from Holoptelea integrifolia (Roxb.) Planch Leaves

Abstract

Objective: The present study was conducted to identify the tannins from petroleum ether (PEHI) and methanol extracts (MHI) of medicinally and economically useful leaves of Holoptelea integrifolia (Roxb.) Planch using High Performance Thin Layer Chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungstant lamp. Results: Preliminary phytochemical screening of petroleum ether extract of Holoptelea integrifolia showed the presence of steroids, terpenoids, alkaloids, glycosides, flavonoids, proteins, tannins and carbohydrates while methanolic extract of Holoptelea integrifolia showed the presence of steroids, alkaloids, flavonoids, tannins, proteins and carbohydrates. HPTLC finger printing of tannins of petroleum ether extract of leaves revealed eleven polyvalent phytoconstituents (11 peaks) and corresponding ascending order of Rf values in the range of 0.20 to 0.75, while methanol extract of leaves showed twelve polyvalent phytoconstituents (12 peaks) and corresponding ascending order of Rf values in the range of 0.12 to 0.73. Conclusions: With the results of preliminary phytochemical analysis and above Rf values we have concluded the presence of tannins in both the extracts.