Induced breeding, egg and embryonic development of Pangasianodon hypophthalmus (Sauvage, 1878) under hatchery conditions of north Tamil Nadu (Chennai)

Abstract

Induced breeding experiments were conducted during mid-August to September 2015 using GnRH based inducing agents (Synthetic hormones) viz., GONOPRO FH and WOVA FH. Of which, the successful release of gametes was observed in 5 experiments. Breeding hapa was used during the experiments. The inducing agents were tried in various in one/two split doses. Incubation time of 12.5-16.0 hours was given and breeders were stripped artificially. The fertilized egg has been adhesive and was treated with tannin. 60-80% of fertilization of eggs was achieved. The diameter of fertilized eggs increased further by 0.2 mm after fertilization. The eggs were allowed to develop in Jar Hatchery maintained with water flow. The egg development up to late C- cell embryo was attained.