Evaluation of efficiency of four DNA extraction methods in Apis mellifera L. (Hymenoptera: Apidae)

Abstract

Quality and quantity of DNA extraction is important in molecular studies. In this research, four methods were evaluated in order to choose the most appropriate way in DNA extraction. DNA extraction methods included the optimal salt, phenol – choloroform, CTAB and CTAB + SDS. The quantity and quality of DNA extracted were compared with spectrophotometry and agarose gel respectively. Results indicated that CTAB+SDS method with the average purity of 1.89 (absorption ratio 260nm/280nm) was better than to other methods for DNA extraction in Apis mellifera.