HPTLC Standardization and Quantification of Rhinacanthus nasutus

Abstract

Background: Ethnobiological status of the plant R.nasutus (Rhinacanthus nasutus) has been analyzed by HPTLC standardizing and quantifying of flavonoids metabolites. Methods: HPTLC method using 10µl of ethanol extract of R.nasutus at a concentration of 10mg/ml along with 10µl of each 1mg/ml standard concentration of Rutin and Quercetin were applied to the pre-coated silica gel F254 HPTLC plates, and the plate was developed in a twin trough TLC chamber saturated with Ethyl acetate: Formic acid: Glacial acetic acid: water (11:1.1:1.1:2.6). Evaluation by using a scanning TLC scanner was carried out in the absorbance mode at 254 and 366 nm and the result was visualized using CAMAG Visualizer. Result: The extract R.nasutus were estimated for the presence of standards Quercetin and Rutin by HPTLC method done by comparing the RF values and peak matching with HPTLC chromatogram and quantified to be Quercetin - 11.6% and Rutin - 5.4% respectively. Conclusion: Standardized criteria for studying the secondary metabolites such as flavonoids with reliable standard methods are often lacking. Efforts are underway to establish a pharmacoepidemiological evidence base regarding safety and practice of Ayurvedic medicines.