Hepatoprotective and antioxidant activity of Odontonema Cuspidatum (Nees) Kuntze against CCl4-Induced Hepatic Injury in Rats

Abstract

Objective: The present investigation deals with evaluation of hepatoprotective and antioxidant activity of total methanol extract and different fractions of Odontonema cuspidatum on the CCl4 induced hepatotoxicity. Material and methods: Albino Wister rats weighing (140±20 g) of either sex were divided into seven groups (n=8). Hepatotoxicity was induced by CCl4 dissolved in olive oil (1:1, 2 ml/kg bw, S.c). Silymarin (100mg/kg) orally was used as standard drug. Test groups received total methanol extract and different fractions (aqueous, n-butanol and n-hexane fractions) of Odontonema cuspidatum aerial partin a dose of 400 mg/kg/day orally along with CCl4. Treatment was given to all the tested groups daily for 5 days. The hepatoprotective effect of Odontonema cuspidatum was evaluated by assessment of biochemical parameters [(AST), (ALT), (ALP) and total bilirubin] and the antioxidant activity in the liver tissue was estimated by measuring the activities of antioxidant enzymes: reduced glutathione (GSH) as well as the level of lipid peroxidation by Malondialdehyde (MDA). Biochemical observations were also supported with histopathological examination of liver section. Results: Odontonema cuspidatum methanol extract (OCME) (400mg/kg) exhibited a highly significant reduction (p