mtDNA cytochrome-b gene assisted genetic diversity study of Catla catla

Abstract

Catla catla is an important carp species found in the river systems of India and its neighbouring countries with a vast scope for commercialization. In order to determine the genetic variation in Catla population collected from different parts of the country (India), the mtDNA cytochrome-b gene was chosen as molecular marker which could provide useful insights into genetic diversity, origin and divergence of the population. In this investigation, 6 haplotypes with 13 polymorphic sites were detected, out of which all 13 were polymorphic singleton sites with no parsimony informative site. The average nucleotide composition of all Catla haplotype sequences was 30.75% Adenine, 27.45% Thymine, 13.41% Guanine and 28.39% Cytosine. The Nucleotide diversity of the haplotypes is 0.008627 ± 0.005855 and the mean number of pair wise differences is 3.666667 ± 2.155097. Clustering of the samples collected from the same river systems into different groups suggests the variability present in the environment