In vitro thrombolytic potentials of methanolic extract of Vigna unguiculata Linn (seed)

Abstract

Inquisition with methanolic extract of Vigna unguiculata Linn (seeds) was carried out to determine the thrombolytic potential of this plant. Plant seeds were extracted with methanol at room temperature. Then concentrated methanolic extract five different concentration (2mg/ml, 4mg/ml, 6mg/ml, 8mg/ml, 10mg/ml) from concentrated methanolic extract was used to evaluate thrombolytic potential of Vigna unguiculata seeds. An easy & rapid methodology (In-vitro Thrombolytic model) was applied to find out their thrombolytic potential where streptokinase and distilled water were employed as positive and negative controls respectively. The plant showed significant clot lysis, i.e. concentrations 12.01 ± 1.50, 16.48 ± 2.31,24.88 ± 1.49,31.24 ± 0.68,40.33 ± 3.64 at 2mg/dl, 4mg/ml, 6mg/ml, 8mg/ml, 10mg/ml respectively, while the standard (streptokinase) and negative control (distilled water) showed 58.41 ± 3.71 and 2.56±1.23% clot lysis respectively. It is clear that Vigna unguiculata Linn (seed) methanolic extract showed thrombolytic activity significantly while comparing with standard. Our present studied suggest that further studies needed to be carried out to get ultimate conclusion of this studies.