Evaluation of the phytochemical constituents in ADJ6, an anti-diabetic polyherbal formulation by GC-MS

Abstract

Background: According to WHO guidelines, standardization and evaluation of herbal drug are very essential to elucidate various properties such as key phytoactive components. Thus it is very vital for the herbal drug preparations to undergo stringent evaluation procedures in order to develop it in to a viable product for the global market.Objective: Our present study focuses on screening of the polyherbal formulation ADJ6 for the presence phytochemical constituents using GC-MS.Materials and Methods: Initially, Phytochemistry of ADJ6 ethanol and diethyl ether extract was determined using TLC plates and sprayer reagents. GC-MS analysis of ADJ6 was performed on GC system coupled with mass spectrometry.Results: The phytochemical analysis showed presence of phenols, aminoacids/proteins, anthrones, tannins and terpenes in ethanolic extract while diethyl extract showed presence of terpenes and sugars. GC-MS Analysis of both the extracts revealed the presence of key phytochemical constituents. A total of 17 compounds were identified 10 and 7 compounds in ethanol & diethyl ether extract respectively.Conclusion: From our present study, it is evident that ADJ6 contains various phytochemical components which possess Antihyperglycemic property. However further studies should be conducted to elucidate the anti-hyperglycemic property of ADJ6.