Isolation and identification of different Rhizospheres fungi of Mansehra region, Pakistan

Abstract

The aim of the current research work was to analyze different fungal species from selected rhizospheres of Mansehra region of Pakistan. The research work was carried out in the Microbiology Laboratory of Hazara University, Mansehra, during April 2016 to August 2016. Soil samples were collected from 6 different locations of Mansehra including Balakoat, Chatar Plan, Dhodial (Hazara University adjacent area), Collage Doraha, Bafa and Oghi. The media used for the isolation and identification included Nutrient agar, Potato Dextrose Agar (PDA) and PDA with peptone. Following inoculation of soil samples, the media was incubated for four days at 25 °C. 10 species of fungal strains were isolated from the soil sample, which included Cladosporium herbarum, Dermatoium spp., Fusarium oxysporum, Mucor hiemalis, Alternaria solani, Alternaria Alternata, Aspergillus flavus and Aspergillus niger. Fungal growth was then purified on nutrient agar medium. The results of this investigation demonstrated that different strains of fungi are present in the soil of Mansehra region.