Enhancement of growth potentials in freshwater prawn Macrobrachium rosenbergii through supplementation of probiotic diets of Bacillus subtilis and Lactobacillus rhamnosus

Abstract

In the present investigation Wild prawns and Hatchery PL raised prawns were fed with two different probiotic diets. The probiotic diets include Bacillus subtilis and Lactobacillus rhamnosus. Feeding trail experiments were conducted for 120 days and growth parameters were monitored through Weight gain, Specific growth rates, Food conversion ratio, Feed efficiency ratio, Percent survival, Muscle somatic index, Protein efficiency ratio, Normalised biomass index, Microbial analysis and activities of Antioxidant enzymes including Superoxide dismutase and Catalase were monitored and recorded. Regular water quality analysis was conducted for several water parameters including temperature, Transparency, Dissolved oxygen content, pH, Conductivity, Turbidity, Total Alkalinity, Nitrite (NO2), Nitrate (NO3),Ammonia (NH3), Total Nitrogen, Total Phosphorous, Chlorophyll-a and Chemical oxygen demand (COD) were monitored and recorded. Feed trail experimental results obtained clearly demonstrate that Probiotic diet (PB-I) containing Lactobacillus rhamnosus Supplementation induces highest growth potentials in terms of weight gain compared to Probiotic diet II (PB-II) containing B.subtilis supplemented and Control group fed with Commercial diet. The FCR values also recorded to be better with PB-I diet compared to PB-II and Control diet, demonstrates the efficient utilization of feed by the prawn M. rosenbergii. The water quality parameters are seems to be more congenial for inducing better growth potentials when PB-I and PB-II are being made available for feeding in the feeding trail experiments. The Antioxidant enzymes represented by Superoxide dismutase and Catalase demonstrates the reduction of ROS in the body of Prawns there by inducing highest and best growth potentials in Freshwater Prawn M. rosenbergii. All the changes are more pronounced in prawns collected from wild source compared to Hatchery raised prawns indicating that the wild source of prawns are showing the better growth rates and efficient mechanisms of detoxification, there by the growth potentials recorded are to be superior to the Hatchery raised prawns. Both the Probiotics selected B. subtilis and L. rhamnosus are considered to be more efficient inducing best and highest rate of growth potentials in Freshwater prawn M. rosenbergii.