Evaluation of antidiabetic activity of bioactive constituent of Sauropus androgynus in alloxan induced diabetic Rats and effect on inhibition of α-glucosidase enzyme

Abstract

The antidiabetic effect of bioactive constituent isolated from alcoholic extract of the leaves of S. androgynus investigated in alloxan induced diabetic rats. The antidiabetic potential tested by docking studies using isolated compound as ligand and intestinal I±-glucosidase as receptor. The isolate and the standard hypoglycaemic drug glibenclamide (10mg/kg) were dissolved in isotonic saline solution (NaCl, 0.9%w/v) and were administered orally. Diabetes was induced in by a single intraperitoneal injection of 150mg/kg alloxan monohydrate. Hypoglycaemia was confirmed by the elevated glucose levels determined at 72 hr after alloxan injection. The rats having blood glucose levels above 200mg/dL were used for the antidiabetic study. The isolate and standard drug were administered orally for 14 days. The fasting blood samples were collected by retro orbital sinus puncture under mild ketamine xylazine anaesthesia. Fasting blood glucose estimation was measured on day 0, 7 and 14 of the study using glucometer. The result reveal that the isolate at both dose levels (200 and 400mg/kg) cause a significant (p < 0.001) decrease in the blood glucose levels after oral administration at 7 and 14 days when compared to the diabetic control group. The isolated compound tested for their antidiabetic potential by docking studies using intestinal I±- glucosidase as receptor. Chemically the isolated compound is a bound flavonoid which binds with the I±-glucosidase with docking score of -9. The drug receptor interaction studies also shows excellent hydrogen bond and hydrophobic interactions. The result indicates that the isolated compound is potent I±-glucosidase inhibitor with good antidiabetic potential.