Antioxidant, cytotoxic and thrombolytic activity of leaves of Kalanchoe pinnata (LAM.) PERS.

Abstract

The study was aimed to evaluate the, antioxidant, cytotoxic and thrombolytic activity of ethanol extract of the leaves of Kalanchoe pinnata. In vitro antioxidant activity of extract was studied using DPPH radical scavenging, reducing power determination assays. The antioxidant activity of the extracts was found promising. The reducing powers of this crude extract increase with the increase of concentration. IC50 values of DPPH scavenging activity was 282.136±0.46μg/ml. The cytotoxic activity of crude extract was determined using brine shrimp lethality bioassay and LC50 values of the sample was 51.88 ± 0.52μg/ml whereas for standard vincristine sulphate was 8.50 ± 0.16μg/ml as a positive control. The plant’s extract showed (15.73 ±1.31%) clot lytic as compared to standard streptokinase’s (30.26% ± 0.67) clot lytic activity in case of thrombolysis assay