Phytochemical and biological investigation of VLC fraction of Senna alata leaves

Abstract

The objective of this work was phytochemical and biological investigation of VLC fraction of Senna alata leaves found in Bangladesh. The crude methanolic extract was fractionated by vacuum liquid chromatography by different five polar solvent. The resulted fractions were again fractionated by vacuum liquid chromatography by same five different polar solvent. We investigated the presence of polyphenols, flavonoids and other types of compounds by thin layer chromatography. In vitro antioxidant effects were measured by DPPH scavenging assay. In vitro anti- diabetic assay was carried out by glucose uptake in yeast cells. Disc diffusion assay was performed to show the antibacterial effect using gram positive, gram negative strains of bacteria and fungi. Receptor binding activities was performed by hemagglutination inhibition assay. VLC fraction of leaves contain flavonoids and other biologically active compounds. The extract showed antioxidant and anti-diabetic activity. The extract also showed antibacterial activities against several strains of bacteria. It also showed hemagglutination inhibition activities in human blood cells. Therefore, leaves of Senna alata may be considered as a source of various health benefits.