Development and characterization of two new cell lines, CGL, and CGK from the liver and kidney of Channa Gachua

Abstract

Two new cell lines (CGL and CGK) are developed from the liver and kidney tissues of C. gachua, (Ham), a freshwater wild fish severely affected with EUS. The tissues were disintegrated mechanically and seeded with 20% fetal bovine serum. The cells showed optimal growth at 25 0 C with Egale's MEM's containing 5% L-glutamine. The major problem in the development of cell line from small size fish is slow growth rate in vitro, which is overcome by supplementing with the supernatant from the conditioned media from the culture of head kidney of the same species. The monolayer is obtained within 20 days of seeding and culture is observed up to 26 passages. The ultra-structural studies of these cell lines showed it’s synthesizing and secretary in nature.