Evaluation of in vitro anticancer activity of stem of Tinospora cordifolia against human breast cancer and Vero cell lines

Abstract

Tinospora cordifolia, also known as Giloy, Guduchi or Amrita, is used in the treatment of various diseases in the traditional medicinal system in India and is also an immune system modulator. In the current study, in vitro cytotoxic activity of 50% methanolic extract of stem of Tinospora cordifolia was evaluated against human breast cancer cell line MDA-MB-231 and normal Vero epithelial cell line. Methanolic extract of Tinospora cordifolia showed significant anticancer activity against MDA-MB-231 human breast cancer cell line. IC50 values of TC methanolic extract with respect to MDA-MB-231 were found to be 59 ± 4.05 μg/ml in 0.25% DMSO and 50± 2.01 μg/ml in 0.5% DMSO. Methanolic extract of Tinospora cordifolia was shown to possess cytotoxic activity against human breast cancer cells. The methanolic extract would be studied further for isolation and characterization of active components for lead optimization studies.