In vitro and in silico effect of λ- Cyhalothrin on serum lactate dehydrogenase in Rattus norvegicus

Abstract

λ- Cyhalothrin belongs to a group of pyrethroid pesticides being used in various pest control programs. Prediction of binding of λ- Cyhalothrin on LDH in Rattus norvegicus was analyzed in the current study, using in silico and in vitro methods. The Molegro Virtual Docker (MVD) was used for in silico study and diagnostic reagent kit for in vitro study. The comparative mole dock score, root-mean square deviation (RMSD), affinity, interacting residues of LDH, number of hydrogen bond interaction, docking score, protein steric interaction energy (Protein EvdW) and the interaction of residues were evaluated with the help of in silico study. The λ- Cyhalothrin binds with the lysine (Lys56) and tyrosine (Tyr 246) and forms three hydrogen bonds with LDH. λ- Cyhalothrin causes dose as well as time dependent toxicity after acute and late sub chronic treatments has been proved after in vitro study.