Standardization and a-glycosidase inhibition of extracts of Vatica pauciflora Blume stem barks and Smallanthus sonchifolius leaves

Abstract

Objective: To investigate standardizations of extract of V. pauciflora and S sonchifolius and to conduct in vitro assay of a-glycosidase enzyme inhibitory effects of the extracts. Methods: Extraction of leaf and stem bark powder was conducted with maceration by using 70% ethanol as solvent, followed by extract quality determination that involved extract specific parameters, such as extract organoleptic assay, ethanol soluble content, water soluble content, heavy metal residue as well as extract non-specific parameters, such as loss on drying, water content, total residue content, and solvent residue. The researchers used in vivo assay for a-glycosidase enzyme inhibitory effects.rnResults: The study showed that ethanolic extracts of V. pauciflora and S sonchifolius could be standardized with specific and non-specific parameters and had a-glycosidase enzyme inhibitory effects, with IC50 values of 19.59 and 13,53 mg/mL.rnrnConclusions: Standardized extract of V. pauciflora and S sonchifolius have a-glycosidase enzyme inhibitory properties.