Quality control study and standardization of Hibiscus rosasinensis l. flowers and leaves as per WHO guidelines

Abstract

Hibiscus rosasinensis flowers and leaves are available all over India and well known for its Antidiabetic property. In the present investigation aqueous extract and ethanolic extract of the flowers and leaves were made using soxhlet apparatus, water extract and maceration. The qualitative Phytochemical screening procedure was performed on each extract. Phytochemical study reveals that alkaloids, tannins, saponins, triterpenoids, coumarins, steroids, flavonoids were present in the three extracts. An attempt has been made to highlight this folk herbal medicine through present study which will assist in the identification of fresh as well as dried crude samples of flowers and leaves anatomically and physico chemically. TLC finger printing and fluorescence analysis of powdered flowers and leaves has been conducted and reported .The antidiabetic activity was performed by enzyme inhibition (I±-glycosidase) in in vitro method on each extract and ethanolic extract showed significant inhibition.