Amylase activity of fingerlings of freshwater fish Labeo rohita fed on formulated feed.

Abstract

In the present study ninety days experiment was conducted in glass aquaria to study amylase activity in intestinal tissue of fingerlings of freshwater fish Labeo rohita fed on 100%, 75%, 50% and 25% nonconventional source of protein in formulation of feed i.e. Blood of bovine animals obtained from slaughter house and conventional feed i.e. groundnut oil cake. The fishes were fed at the rate of 2% of the body weight every day. After specific time intervals the fishes were weighed and sacrificed for intestinal tissue. The intestinal tissues were quickly excised and cleaned off extraneous material, weight and used for enzymatic estimation of amylase by Bern Field (1955) method. Results revealed that the amylase activity was highest at 75% followed by 50%, 25% and 100% formulated feeds as compared to conventional feed.