A Newer Validated and Stability Indicating GC Method for the Estimation of Lindane in Formulation

Abstract

A simple, selective, linear, precise and accurate GC Method was developed and validated for rapid assay of Lindane in Formulation. Isocratic elution at a flow rate of 1.0ml/min was employed on DB 1,30 m × 0.53 mm Capillary column at ambient temperature250 °C. Injection Volume was found to be 2 µl. The mobile phase consisted of Acetone: Chloroform 80:20 v/v. The UV detection wavelength was 240nm and 2µl sample was injected. The retention time for Lindane was 6.2 min. A linear regression curve was constructed, and the correlation coefficients (R 2 ) and assessment values calculated The percentage RSD was found to be 1.0%.The Accuracy of method ranges between 97.0 – 102%. The method was validated as per the ICH guidelines. The method was successfully applied for routine quality control analysis of pharmaceutical formulation. Keyword: Lindane, GC, Recovery, Precise.