Part Based HPLC-PDA Quantification of Podophyllotoxin in Populations of Podophyllum hexandrum Royle "Indian Mayapple" from Higher Altitude Himalayas

Abstract

In the present study, a simple, sensitive, selective and reliable HPLC method based on photo diode array detector (PDA) has been developed and validated for the simultaneous determination of an important compound (podophyllotoxin) in leaves and rhizomes parts of four different populations of Podophyllum hexandrum Royle from North Indian Himachal Pradesh higher altitude Himalayas. The analysis was carried out on a RP-18e (LiCrosphere, 5µm, 250 x 4.0 mm) column, with isocratic elution of acetonitrile: water (40:60, v/v). The method was validated for accuracy, precision, limit of detection and quantification. The regression equation revealed a good linear relationship (r2 = 0.9999 for podophyllotoxin) within test ranges. The limit of detection and limit of quantification for analyte in PDA was 50 and 110µg, respectively. The method showed good reproducibility for the quantification of podophyllotoxin in P. hexandrum. Keyword: HPLC-PDA, Podophyllotoxin, Indian Mayapple, Podophyllum hexandrum Royle, Himachal Pradesh, Higher altitude Himalayas.