Review on biological activity and determination of E&Z- Guggulsterones concentration by HPLC & HPTLC Methods

Abstract

The resinous exudates of guggul known as gum-resin have important therapeutic properties reported in ancient Indian Ayurveda system. The guggul gum-resin used for the treatment of different diseases such as obesity, liver disorder, ulcers, inflammatory, cancer. An ethyl acetate extract of gum-resin known as ‘guggulipid’ contains hypolipidemic agents two ketosteroids  E&Z-guggulsterones.Guggulsterones exhibited different biological activities such as anticholesterol, antidiabetic, anti-inflammatory, anticancer and hepatoprotective. HPLC and HPTLC method effectively separate both isomers and method validated to determine the concentration of guggulsterones. Result showed that that ratio of guggulsterones vary with different geographical area and different climate condition. and also it was also different from claimed in the marketed products. In this review covers literature on HPLC and HPTLC method of separation and estimation of guggulsterones.